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. 2018 Sep 21;9:238. doi: 10.1186/s13287-018-0984-0

Fig. 6.

Fig. 6

HUVEC migration measured using xCELLigence real-time cell analyser. After 24 h, migration of HUVECs cultured with 100 mM glucose (100(in)) significantly reduced as compared to glucose-untreated HUVECs (a). As compared to glucose-untreated HUVECs, HUVEC migration in presence of glucose and CMpMSC (100 + CM(in)) did not significantly change (p > 0.05) after 24 h but migration significantly increased as compared to glucose-treated HUVECs (100(in)) (a). Migration of HUVECs in response to 100 mM glucose alone (100(out)), or with CMpMSC (100 + CM(out)) added to lower chamber of migration plate, significantly reduced as compared to glucose-untreated HUVECs after 24 h (b). As compared to glucose-treated HUVECs (100(out)), HUVEC migration in response to glucose and CMpMSC (100 + CM(out)) did not significantly change after 24 h as compared to glucose-treated HUVECs (100(out)) (b). After 24 h, migration of HUVECs pretreated with glucose alone (100(pre), or with glucose and CMpMSC (100 + CM(Pre)), or with glucose and ICpMSC (100 + IC(Pre)), significantly increased as compared to glucose-treated HUVECs (100(pre)) (c). After 24 h, as compared to glucose-treated HUVECs (100(pre)), migration of HUVECs pretreated with 100 mM glucose and CMpMSC (100 + CM(Pre)), or with 100 mM glucose and ICpMSC (100 + IC(Pre)), did not significantly change (p > 0.05) (c). Each experiment performed in triplicate using HUVECs (passage 3–5) and pMSCs (passage 2) from five independent umbilical cord tissues and placentae, respectively. *P value is significant < 0.05. Bars represent standard errors