Fig. 9.

Requirement of RelB for the activation of latent HIV-1 in J-Lat A72 cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10⁶) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized