Table 1.

Duplication rate of libraries prepared by different kits

Kit	Sample type	PCR cycles for lib prep	Percent_Duplication (%)
			replicate			avg
			1	2	3
NEBa	Input	22	97	99	97	97
	IP		99	99	99	99
NuG/Trub	Input	8	52	53	52	53
	IP		36	31	26	31
SMTerc	Input	15	83	86	85	85
	IP		86	83	68	79
Tru4d	Input	22	67	77	71	72
	IP		85	82	99	89
Tru70e	Input	19	90	94	92	92
	IP		98	97	96	97
SMTseq4f	Input	11	40	38	40	40
	IP		60	49	48	52
SMTseq0.25f	Input	11	37	37	41	38
	IP		59	50	47	52

^aNEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina

^bNugen Ovation® RNAseq System v2

^cSMARTer® Stranded Total RNA-Seq Kit-Pico Input Mammalian User

^dTruSeq® RNA sample preparation v2,4 ng

^eTruSeq® RNA sample preparation v2,70 ng

^fSMART-Seq v4 Ultra Low Input RNA Kit