Fig. 6.

The expression of KARs in the cultured cortical neurons from Fmr1 WT and Fmr1 KO mice. a, b, The abundance of GluK1 or GluK2/3 in the homogenate of the cultured cortical neurons from Fmr1 WT mice and Fmr1 KO mice showed no change. c, d, Surface expression levels of GluK2/3 in insular cortex neurons obtained from Fmr1 WT and Fmr1 KO mice were detected by western blot analysis. The surface expression levels of GluK2/3 were not altered between Fmr1 WT and Fmr1 KO mice (n = 3 independent experiments). Actin was used as negative control for surface biotinylation