Fig. 7.

Activity-induced internalization of GluK2 subunit was impaired in cultured cortical neurons from Fmr1 KO mice. a, b, GluK2/3 subunit undergoes activity induced internalization in the cultured rat cortical neurons (n = 5 independent experiments). *P < 0.05. c, d, The cultured cortical neurons obtained from Fmr1 WT or Fmr1 KO mice were activated by KA (10 μM) for 20 min and the surface expression of GluK2 was detected by Western blot. The abundance of surface GluK2 in Fmr1 WT mice was significantly decreased upon KA stimulation, whereas that of GluK2 in Fmr1 KO mice did not change (n = 4 independent experiments). *P < 0.05