Table 2. Primer sequences, repeat motif, size range of alleles, and annealing temperature (Tm) of 11 polymorphic EST-SSR markers developed for T. grandis.
| Locus | Primer pair (5′–3′) | Repeat motif | Size range (bp) | Tm (°C) |
|---|---|---|---|---|
| GR12 | F: GCTGTCGAAGCGTTGGAGAA | (ATTT)6 | 204–216 | 56 |
| R: TCTGAAACCTCGCTCGAACC | ||||
| GR16 | F: GTCGCATGCACCTGTTCTTG | (TGCTA)5 | 140–150 | 57 |
| R: GCATAGCGGGGTGAATGAGT | ||||
| GR28 | F: AATGGGAAATTTGGCAGGCG | (ACAGGC)8 | 290–314 | 56 |
| R: GGCCTTCACCACAGCTATGT | ||||
| GR29 | F: CGTCAACGAGGGCAAAAAGG | (CAGAAC)9 | 122–152 | 56 |
| R: TTCTCTCCTACCGCCTTCGA | ||||
| GR34 | F: GCTTGCGCGGATGTAAACAT | (GGGAGT)6 | 119–137 | 57 |
| R: GCGCAGAGCTTTCCAGTAAA | ||||
| GR44 | F: TGATCTCAAGGGGGACTGCA | (GA)9 | 216–220 | 58 |
| R: CTTAGTATGCAGCCGAGGCA | ||||
| GR48 | F: TTTTAGAACTGCTTGCCCGT | (CA)11 | 197–205 | 58 |
| R: CATGTACATGCACCATCATGC | ||||
| GR67 | F: TCCAGTCAGCGCGAATAGTC | (TCC)12 | 141–162 | 58 |
| R: AGTAGAGGAGTCCATGGCGT | ||||
| GR80 | F: AGCAGCATGGAGGACGATTC | (GCC)13 | 197–224 | 57 |
| R: CCTCGGGCATGTATCCATCC | ||||
| GR81 | F: GGCTCAGTACTCCCAAACCC | (CCT)7 | 211–226 | 57 |
| R: TCGGCTCCTTTATACGACGC | ||||
| GR98 | F: TATTCGAGACGCGCATTCGA | (ATCT)5 | 161–173 | 58 |
| R: CTCGCATTGAAGCTGTCTGC |