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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Virol Methods. 2018 Aug 17;261:91–97. doi: 10.1016/j.jviromet.2018.08.009

Table 3:

Comparison of HIV-1 TNA Concentration in BLD and DBS Specimens

TYPE HIV-1 TNA copies Dl1 D10
n= Geometric Mean MIN MAX n= Geometric Mean MIN MAX p-value
BLD 36 3,128 303 15,972 36 2,660 216 16,777 0.392
DBS 36 994 57 9,057 ND ND ND ND <0.0013
1

The geometric mean, miminum (MIN), and maximum (MAX) HIV-1 TNA concentration in 36 samples tested at day 1 (frozen whole blood [BLD], dried blood spots [DBS]) and day 10 (BLD) after collection. HIV-1 TNA concentration was measured using a modified Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 RNA assay (section 3.4).

2

BLD HIV-1 TNA copies/0.1mL created on the same day of collection (D1) vs. BLD HIV-1 TNA copies/0.1 mL from samples created after ten days of storage (D10); p-value derived from mixed model using day as fixed factor and panel as random factor (interaction of day * panel, p = 0.95); note fold change from Day 1 to Day 10 in BLD= 0.85.

3

BLD HIV-1 TNA copies/0.1 mL for D1 testing vs. DBS HIV-1 TNA copies/spot (0.075 mL) from D1 testing; p-value derived from mixed model using sample type as fixed factor and panel as random factor (interaction of sample type * panel, p = 0.14); note fold difference between DBS vs BLD is 0.32.