| Antibodies |
| Goat polyclonal anti-Glutamine Synthetase |
Abcam |
Cat# ab6585 |
| Mouse monoclonal Anti-E coli RNA Sigma 54 (clone 6RN3) |
BioLegend |
Cat# 663303 |
| Mouse monoclonal anti-GAPDH (clone GA1R) |
Thermo Fisher |
Cat# MA5-15738 |
|
|
|
| Bacterial and Virus Strains |
|
E. coli: MG1655 |
Coli Genetic Stock Center – Yale University |
Cat# CGSC 6300 |
|
E. coli: BW25113 |
Coli Genetic Stock Center – Yale University |
Cat# CGSC 7636 |
|
E. coli: Rosetta 2 (DE3) |
Novagen |
Cat# 71397 |
|
|
|
| Chemicals, Peptides, and Recombinant Proteins |
| B-PER II |
Thermo Fisher |
Cat# 78260 |
| 2-methylimidazole |
Alfa Aesar |
Cat# A11949 |
| 2,4-dimethylimidazole |
Sigma Aldrich |
Cat# M50850 |
| Adenosine diphosphate sodium salt |
Sigma Aldrich |
Cat# A2754 |
| Hydroxylamine Hydrochloride |
Sigma Aldrich |
Cat# 159417 |
| Sodium Tartrate |
Sigma Aldrich |
Cat# SX0795 |
| Cobalt chloride hexahydrate |
Sigma Aldrich |
Cat# C2644 |
| Sodium molybdate dihydrate |
Sigma Aldrich |
Cat# 221848 |
| Copper chloride dehydrate |
Sigma Aldrich |
Cat# 459097 |
| SYBR Select Master Mix |
Applied Biosystems |
Cat# 4472942 |
|
|
|
| Critical Commercial Assays |
| CellTiter-Glo |
Promega |
Cat# G7571 |
| BacTiter-Glo |
Promega |
Cat# G8231 |
| Ammonia Quantification Assay |
Sigma Aldrich |
Cat# AA0100 |
| RNeasy Mini Kit |
Qiagen |
Cat# 74106 |
| Superscript III First strand cDNA synthesis system |
Invitrogen |
Cat# 18080051 |
|
|
|
| Deposited Data |
| Deep sequencing data |
GEO |
Acc# GSE111317
|
|
|
|
| Experimental Models: Cell Lines |
| Human: WS1 Fibroblast cells |
ATCC |
Cat# CRL-1502 |
|
|
|
| Oligonucleotides |
| See Table S1 for DNA oligo and primer sequences |
This study |
N/A |
|
|
|
| Software and Algorithms |
| GraphPad Prism 7.01 |
GraphPad Software |
https://www.graphpad.com/ |
| SnapGene 3.0.3 |
GSL Biotech |
www.snapgene.com |
| ImageJ |
National Institutes of Health |
https://imagej.net/NIH_Image |
| ChemDraw 15.1 |
PerkinElmer |
http://www.cambridgesoft.com/software/overview.aspx |
