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. Author manuscript; available in PMC: 2018 Oct 5.
Published in final edited form as: Leukemia. 2018 Mar 22;32(10):2250–2262. doi: 10.1038/s41375-018-0104-2

Fig. 5. MALAT1 coordinated with PAPR1 inhibitor through inhibiting A-NHEJ.

Fig. 5

(A) In A-NHEJ reporter plasmid (pEJ2-GFP-puro) stable expression 293T cells, pCBA-SceI was transient transfected with MALAT1 overexpression/empty vector or anti-MALAT1/control gapmer. Then GFP positive cells were determined by flow cytometry. (B) The co-localization between LIG3 and PARP1 or γH2A.X were determined by immunofluorescence staining (scale bar=5μM). (C) H929, MM.1S and RPMI8226 cells transfected with anti-MALAT1 or control oligos were treated with ABT-888. Cells were collected for WB. MALAT1 were determined by qRT-PCR.(*p<0.05, **p<0.01)