Figure 1.

Quanlity assessment of BSA-AGEs and their binding to cells. (a) Full-wavelength scanning was performed to analyze the fluorescent density of quanlity of the prepared AGEs, which indicated the aunlity of the preparation. Excitation wavelength was 370 nm. (b) Cells were treated with AGEs for 4 hours and stained with indirect immunofluorescence staining against BSA and then measured by flow cytometry, which indicated the cell binding of AGE. Every experiment was repeated at least three times.