Table 1. Universal primer pairs used to target the selected regions of the COI and 18S genes.

The COI primers, mlCOIintF and jgHCO2198, target a 313 base pair (bp) region. The 18S rRNA primers, Uni18SF and Uni18SR, target a 425 bp V4 variable region. Illumina Nextera™  library adapters (NexAd) have been added to the primers and are underlined.

Primer Name	Target	Sequence(adapters underlined)	GC%
mlCOIintF_NexAd (Forward)	COI 313 bp region	5′ TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG GGW ACW GGW TGA ACW GTW TAY CCY CC 3′	48.3
jgHCO2198_NexAd (Reverse)	COI 313 bp region	5′ GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA G TA IAC YTC IGG RTG ICC RAA RAA YCA 3′	42.6
Uni18SF_NexAd (Forward)	18S V4 425 bp region	5′ TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG AGG GCA AKY CTG GTG CCA GC 3′	54.7
Uni18SR_NexAd (Reverse)	18S V4 425 bp region	5′ GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA G GR CGG TAT CTR ATC GYC TT 3′	49.1