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Antibodies used.
| Primary antibody | Specification | Dilution | Source |
|---|---|---|---|
| β-actin | Monoclonal mouse | 1:5000 | Sigma–Aldrich |
| EGFR | Monoclonal rabbit | 1:1000 | Cell Signaling |
| VEGFR-1 | Polyclonal rabbit | 1:1000 | Cell Signaling |
| PDGFRβ | Monoclonal rabbit | 1:1000 | Cell Signaling |
| ERK1/2 (p44/42 MAPK) | Polyclonal rabbit | 1:1000 | Cell Signaling |
| pERK (Thr202/Tyr204) | Polyclonal rabbit | 1:1000 | Cell Signaling |
