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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Kidney Int. 2018 Aug 6;94(4):756–772. doi: 10.1016/j.kint.2018.05.023

Figure 8.

Figure 8

XL-001 inhibits renal expression of pro-inflammatory cytokines and reduces renal inflammation after UUO. (a–d) qRT-PCR shows the relative abundances of renal TNF-α (a), MCP-1 (b), IL-6 (c), and RANTES (d) mRNA in different groups as indicated. *P < 0.05 versus sham controls. †P < 0.05 versus UUO alone (n = 5 to 6). The doses of XL-001 (0, 10, 20 mg/kg body weight) were indicated. (e) Representative micrographs show renal infiltration of CD3+ T cells and MR+ macrophages in different groups as indicated. Paraffin-embedded kidney sections were stained with antibodies against RANTES. Arrow indicates positive staining. Scale bar, 50 μm. (f) Western blot analyses show renal levels of the phosphorylated-p65 (p-p65) and total p65 in different groups as indicated. Numbers (1 to 3) represent different individual animals in a given group. (g, h) Graphic presentations show the relative protein levels of renal p-p65 (g) and total p65 (h) in different groups. †P < 0.05 versus UUO alone.