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. 2018 Sep 6;175(20):3928–3946. doi: 10.1111/bph.14465

Figure 6.

Figure 6

Activated c‐Src, Pyk2 and Akt play crucial roles in rosiglitazone‐induced HO‐1 expression. (A) The cells were incubated with various concentrations of c‐Srci II, PF431396 or Akti VIII for 1 h and then stimulated by vehicle or rosiglitazone (30 μM) for 16 h. The levels of HO‐1 and GAPDH protein were determined by Western blot. (B) The cells were pretreated with c‐Srci II (10 μM), PF431396 (10 μM) or Akti VIII (10 μM) for 1 h and then incubated with vehicle or rosiglitazone (30 μM) for 8 h. The levels of HO‐1 and GAPDH mRNA were determined by real‐time PCR. (C) HPAEpiCs were transfected with c‐Src, Pyk2 or Akt siRNA and then incubated with rosiglitazone for 16 h. The levels of HO‐1, c‐Src, Pyk2 and GAPDH protein were determined by Western blot. Data are expressed as mean ± SEM, from five independent experiments (n = 5). # P < 0.05, significantly different from rosiglitazone alone (A, B); or significantly different as indicated (C).