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L5 peptide binding assays. HepG2 cells and HL-7702 cells were incubated with either FAM (a, b) or L5-FAM (c, d), respectively, which was mounted with DAPI-containing media (e, f). Excess L5 peptide blocked the binding of HepG2 cells with L5-FAM, exemplifying competitive binding and strong affinity of L5 peptide to GPC3 (g).
