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. 2017 Oct 23;22(10):1792. doi: 10.3390/molecules22101792

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Chromatogram of the High Performance Liquid Chromatography with a Diode-Array Detector (HPLC-DAD) analysis of the investigated G5 fraction from Schinus terebinthifolius Raddi fruit extract (4.0 mg/mL) at wavelength of 254 nm. Analytical conditions: stationary phase: RP-18 reverse-phase column (5 μm, 250 mm, 4.5 mm, Macherey-Nagel); temperature: 32 °C; mobile phase: A = purified water adjusted to pH 3.2 with phosphoric acid, B = acetonitrile; flow rate: 1.0 mL/min; detection: 254 nm; injection volume: 20 μL; solvent composition during analysis: 0′: 100% A; 5′: 70% A; 10′: 50% A; 15′: 30% A; 20′: 20% A; 25′: 10% A; 30′: 0% A; 32′: stop.