Table 3.

Mixing ratio of six standards in each mixture.

Mixtures b	Standards (μg/mL) a
	1	2	3	4	5	6
1	EC5	EC10	EC20	EC30	EC40	EC50
2	EC10	EC30	EC50	EC5	EC20	EC40
3	EC20	EC50	EC10	EC40	EC5	EC30
4	EC30	EC5	EC40	EC10	EC50	EC20
5	EC40	EC20	EC5	EC50	EC30	EC10
6	EC50	EC40	EC30	EC20	EC10	EC5
7	EC50	EC50	EC50	EC50	EC50	EC50
8	EC5	EC5	EC5	EC5	EC5	EC5

^a Standards 1, 2, 3, 4, 5 and 6 were epimedin A, epimedin B, epimedin C, icariin, baohuoside I and icartin, respectively; ^b mixtures 1–6 were composed of fixed concentration ratios of six standards through uniform design, and mixtures 7 and 8 were composed of fixed concentration ratios of six standards through the equivalent concentration method.