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Effect of paradol on mRNA expression of some ossification-related genes in SAOS-2 cell line. Cells were incubated with paradol (1 uM) or E2 (0.1 uM) for 48 h. Total RNA was extracted and subjected to RT-PCR. Data were normalized to β-actin; fold changes were calculated and expressed as mean ± SD; n = 3. * Significantly different from control untreated cells; p < 0.05.
