Figure 4.

TNF levels in supernatants of cultured J774A.1 murine macrophage cells after exposure to amphiphilic lipopeptides containing different lipid chains, but all possessing the same S-F-R linear peptide headgroup. Test substances were placed in the wells four hours prior to stimulation by CTB or LPS. TNF levels were measured 18 h after stimulation of the macrophages. Moz 2-3 contains two C12 lipidic amino acids as anchors. Moz 3-3 contains a single C12 amino acid, while Moz 5-1 contains a single C14 amino acid.