The effect of ZJF pretreatment on the nuclear factor kappa B p65 (NF-κB p65), nuclear factor erythroid 2-related factor 2 (Nrf-2), and NAD(P)H: quinone oxidoreductase 1 (NQO1 ) protein expression in response to the APAP-induced hepatotoxicity. (A) Western blotting analyses of NF-κB p65, Nrf-2, and NQO1 proteins; quantitative densitometric analyses of (B) NF-κB p65, (C) Nrf-2, and (D) NQO1 proteins normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Each value represents the mean ± SEM of three independent experiments. ## p< 0.01 vs. the control group. * p < 0.05 and ** p < 0.01 vs. the APAP treated group.