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. 2017 Oct 17;22(10):1705. doi: 10.3390/molecules22101705

Figure 2.

Figure 2

Effect of AF/AD on apoptosis of Bel-7402 cells with or without SB203580 or 3-MA. Bel-7402 cells were pretreated with AF (6 μM)/AD (20 μM) in the absence or presence of SB203580 (10 μM) or 3-MA (5 mM) for 24 h, and then the percentage of apoptotic cells was evaluated by flow cytometry analysis of annexin V-FLUOS/PI double-stained cells. Values presented are representative of three independent experiments (means ± S.D.; ** p < 0.01, compared with control treatment; ## p < 0.01, compared with AF/AD treatment).