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HMGB1 release from irradiated cancer cells. Three human cancer cell lines were treated with a D10 dose of 13 or 70 keV/μm C-ion beams and cultured for the indicated times. ELISA was used to measure the HMGB1 concentrations in the culture supernatants. The results are expressed as the mean ± SD of four independent experiments. *, P < 0.05;**, P < 0.01;***, P < 0.001.
