Figure 1.

The Minimal Inhibitory Concentration (MIC) was established using the macrodilution method (n = 3) according to the Clinical and Laboratory Standard Institute (CLSI) standard M07-A9. A significant decrease in bacterial growth after 24 h was detected when totarol concentrations of 64 µg/mL or higher were used. Data are given as means with standard error of the mean (SEM). One-way RM (repeated-measures) ANOVA followed by Tukey’s multiple comparisons test was used to identify significant differences between the varied totarol concentrations. **** indicates that the results are significantly different compared to the EtAc control (p < 0.0001). The white bar shows the growth control; the light grey bar depicts bacterial growth in the presents of EtAc and the dark grey bars display the growth of S. aureus exposed to different concentrations of totarol.