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. 2017 Sep 8;22(9):1502. doi: 10.3390/molecules22091502

Figure 3.

Figure 3

(I) Protective effects in N2 worms pre-treated with S. singueana on the survival rate of wild-type nematodes treated with a lethal dose of 80 μM juglone. Significant increase in the survival rate was observed after the worms were pre-treated with the extract. Data are presented as percentage of survival (mean ± SEM, n=3) (II): Intracellular ROS levels in wild-type N2 nematodes evidenced by the fluorescence H2DCF-DA dye. Results are presented as relative fluorescent intensity related to control of the mean ± SEM of three individual experiments in which at least 20 worms were analyzed. The accumulation of mitochondrial ROS was significantly diminished when worms were pre-treated with the extract (III): Effects of the root extract on the translocation of DAF-16 transcription factor in mutant TJ356 strains. Subcellular localization of DAF-16:GFP was analyzed by fluorescence microscopy (200×) and the nematodes were sorted to the categories cytosolic, intermediate, and nuclear according to their localisation phenotype as shown in the representative images.* p < 0.05, ** p < 0.01,*** p < 0.001 related to control was analysed by one-way ANOVA.