Table 3.
Precision, accuracy, extraction recovery, matrix effect, and stability of the seven components in rat plasma samples (n = 6).
| Components | Concentration (ng mL−1) | Inter-Day | Intra-Day | Extraction Recovery (%, Mean ± SD) | Matrix Effect (%, Mean ± SD) | Stability (RE/RSD%) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Precision (RSD%) | Accuracy (RE%) | Precision (RSD%) | Accuracy (RE%) | Short-Term | Three Freeze-Thaw Cycles | Long-Term | ||||
| 1 | 180 | 1.15 | 0.13 | 1.52 | −0.40 | 86.83 ± 3.04 | 88.99 ± 1.86 | 0.55/1.25 | −0.01/1.26 | 1.12/1.40 |
| 22.5 | 1.72 | 0.58 | 2.39 | −0.16 | 93.79 ± 3.79 | 87.06 ± 1.91 | 0.50/1.09 | 1.09/1.25 | 0.59/2.04 | |
| 2.25 | 4.04 | −1.31 | 3.52 | 1.52 | 88.13 ± 3.10 | 88.99 ± 3.82 | 2.15/5.19 | −1.01/3.96 | 0.41/5.29 | |
| 2 | 183.2 | 1.88 | 0.77 | 1.24 | 0.23 | 91.42 ± 5.86 | 88.44 ± 2.98 | 1.12/1.93 | 0.27/2.28 | 1.28/1.72 |
| 22.9 | 2.56 | −0.82 | 3.38 | −1.01 | 93.42 ± 5.63 | 86.91 ± 0.91 | −2.01/3.12 | −2.78/2.73 | −3.21/2.92 | |
| 2.29 | 5.42 | −4.27 | 4.22 | −1.18 | 88.56 ± 1.70 | 87.56 ± 1.44 | −6.50/8.48 | −6.97/8.76 | −5.68/7.73 | |
| 3 | 852 | 1.84 | 1.69 | 1.74 | 2.11 | 87.76 ± 2.10 | 86.18 ± 2.11 | 1.64/1.62 | 1.87/2.27 | 1.78/1.94 |
| 106.5 | 0.73 | −2.97 | 3.62 | −1.02 | 88.56 ± 0.91 | 89.83 ± 4.58 | −0.53/3.65 | −0.09/4.43 | 1.04/2.04 | |
| 2.13 | 3.67 | 1.02 | 3.60 | 0.37 | 95.72 ± 2.47 | 87.29 ± 1.99 | −2.27/7.63 | −2.22/8.02 | −6.33/8.79 | |
| 4 | 548 | 1.83 | −0.03 | 2.49 | 0.85 | 87.72 ± 2.63 | 85.97 ± 1.51 | 2.18/2.14 | 1.26/2.61 | 1.51/2.76 |
| 68.5 | 4.70 | 2.53 | 2.63 | 3.05 | 87.51 ± 1.66 | 87.24 ± 1.95 | 0.28/4.30 | 1.95/3.17 | 3.60/3.68 | |
| 2.74 | 4.19 | −1.00 | 6.34 | −3.22 | 89.91 ± 2.84 | 85.08 ± 7.66 | −0.11/6.65 | −3.67/9.31 | −6.24/7.05 | |
| 5 | 720 | 1.40 | 0.49 | 2.18 | −0.53 | 86.68 ± 1.62 | 86.92 ± 3.29 | 1.27/2.22 | −1.20/1.99 | 0.73/2.14 |
| 90 | 1.47 | 2.33 | 3.62 | −0.16 | 86.39 ± 5.10 | 88.07 ± 3.18 | 0.11/2.56 | 2.76/1.28 | 0.74/3.72 | |
| 9 | 2.47 | 2.10 | 4.86 | 0.45 | 87.03 ± 3.94 | 86.02 ± 1.96 | 14.13/7.65 | −9.21/7.66 | −0.43/7.72 | |
| 6 | 196.8 | 1.85 | 0.69 | 1.04 | 1.52 | 88.68 ± 2.00 | 89.15 ± 4.60 | 1.23/1.64 | 2.18/1.13 | 0.89/2.03 |
| 24.6 | 2.04 | 0.64 | 2.90 | −0.09 | 88.42 ± 0.82 | 85.77 ± 2.99 | 0.48/3.14 | −2.12/3.72 | 0.60/3.10 | |
| 2.46 | 3.40 | −0.75 | 5.42 | −0.13 | 87.45 ± 1.55 | 87.67 ± 3.36 | 2.41/6.57 | −8.06/7.65 | 0.96/9.26 | |
| 7 | 308.8 | 2.14 | 0.67 | 2.59 | 0.62 | 90.20 ± 0.86 | 86.28 ± 2.03 | 0.04/1.00 | 0.59/1.67 | 0.39/1.23 |
| 38.6 | 2.08 | 2.15 | 2.56 | 0.84 | 88.30 ± 1.09 | 86.73 ± 4.23 | 1.60/1.90 | 1.84/2.46 | 1.33/2.07 | |
| 1.93 | 6.50 | 1.25 | 5.26 | −1.25 | 87.61 ± 0.62 | 89.80 ± 8.21 | 3.17/9.21 | 7.58/8.45 | 4.13/6.92 | |