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. 2017 Oct 7;22(10):1669. doi: 10.3390/molecules22101669

Figure 6.

Figure 6

(A) Effect of wortmannin (W) on rosmarinic acid (RA)-induced glucose uptake. L6 myotubes were incubated in the absence (control, C) or presence of 100 nM wortmannin (W) for 15 min followed by the addition of 5.0 μM RA (4 h), 5 μg/mL of RE (4 h) or 100 nM insulin (30 min) and glucose uptake measurements. Results are the mean SE of 6–7 independent experiments. *** p < 0.001 vs. control. ### p < 0.001 vs. treatment in the absence of wortmannin. (B) and (C) Effect of rosmarinic acid (RA) on Akt and P70 S6K phosphorylation. L6 myotubes were treated without (control, C) or with 5.0 μM RA (2 h), I 100 nM (30 min) or 2 mM metformin (MET) (2 h). Whole-cell lysates were prepared, resolved by SDS-PAGE and immunoblotted with specific antibodies that recognize phosphorylated Akt (P-Akt) or total Akt (T-Akt) (B) or phosphorylated p70 S6K (P-p70S6K) or total p70 S6K (T-p70S6K) (C).