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. 2017 Oct 7;22(10):1669. doi: 10.3390/molecules22101669

Figure 7.

Figure 7

(A) Effect of bisindolylmaleimide I (BMD) on rosmarinic acid (RA)-induced glucose transport. L6 myotubes were incubated in the absence (control, C) or presence of 1 μM bisindolylmaleimide I (BMD) for 1 h followed by the addition of 5.0 μM RA (4 h) and glucose uptake measurements. Results are the mean ± SE of 3 independent experiments. *** p < 0.001 vs. control. (B) Effect of RA on PKC phosphorylation. Cells were incubated in the absence or presence of 1 μM BMD for 1 h followed by addition of 5.0 μM RA (2 h) or 200 nM 12-O-tetradecanoylphorbol-13-acetate (TPA) (20 min). Whole-cell lysates were prepared, resolved by SDS-PAGE and immunoblotted with specific antibodies that recognize phosphorylated/activated PKC.