Table 1.
The primer and probe sequences for microarray.
| Targeted gene | Primer/Probe | Sequencing (5'-3') |
|---|---|---|
| CYP450 | 2C192-Fa | CAGAGCTTGGCATATTGTATCT |
| 2C192-R b | GATCAGGAAGCAATCAATAAA | |
| 2C193-F | GATCAGCAATTTCTTAACTTGATGGA | |
| 2C193-R | TTCAAAAATGTACTTCAGGGCTTGGT | |
| 2C192W c | ATTATTTCCCGGGAACCCAT | |
| 2C192M d | TTATTTCCCAGGAACCCAT | |
| 2C193W | TAAGCACCCCCTGGATCCAGGT | |
| 2C193M | GTAAGCACCCCCTGAATCCAGG | |
|
| ||
| 23S rRNA | H. PYLORI23S-F | ATTCAGTGAAATTGTAGTGGAGGTGA |
| H. PYLORI23S-R | CCATTAGCAGTGCTAAGTTGTAGTA | |
| 42W43W | CGGCAAGACGGAAAGACCCC | |
| 42M43W | CGGCAAGACGGGAAGACCCC | |
| 42W43M | GGCAAGACGGAGAGACCCCG | |
| 42M43M | CGGCAAGACGGGGAGACCCC | |
|
| ||
| gyrA | GF240 | GGGTGATGTGATTGGTAAATACCAC |
| GR319 | TTCGAAAAATCTTGCGCCATTCTC | |
| 87WT | CCATGGCGATAATGCGGTTTA | |
| 87WC | CCATGGCGATAACGCGGTTTA | |
| 87MA | CCATGGCGATAAAGCGGTTTA | |
| 87MG | CCATGGCGATAAGGCGGTTTA | |
| 91W | GCGGTTTATGATGCRCTAG | |
| 91MG | TGCGGTTTATGGTGCRCTAG | |
| 91MA | TGCGGTTTATAATGCRCTAT | |
|
| ||
| Quality control e | TTTTTTTTTTTTTTTTTTTT | |
a F: forward primer; b R: reverse primer; reverse primers used for microarray have biotin conjugated at 5'-ends; c W: wild genotype probe; d M: mutant genotype probe, an oligonucleotide of 12 T's with an amino-labeled 3'-end was conjugated to the 3'-ends of all probes; e an oligonucleotide of 20 T's with an amino-labeled 3'-end, biotin-labeled 5'-end was used as microarray quality control.