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. Author manuscript; available in PMC: 2018 Sep 24.
Published in final edited form as: N Engl J Med. 1992 Feb 13;326(7):444–449. doi: 10.1056/NEJM199202133260704

Figure 5. Tth 111 I Digestion of the 800-bp Amplified PrP Coding Region in Samples from Six Members of the Kindred with Fatal Familial Insomnia.

Figure 5.

Three bands of 800, 556, and 244 bp are present in members carrying the GAO→AAC mutation, which abolishes the Tth 111 I restriction site in one of the two alleles (lanes 1, 2, and 3). Only two fragments of 556 and 244 bp are present in members without the mutation (lanes 4, 5, and 6), since both alleles are completely digested (these members are not identified, to protect confidentiality). Lane 7 represents DNA markers — i.e., the products of Bg/l-digested and H/nfl-digested pBR322 DNA.