Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 25;10(438):eaal1803. doi: 10.1126/scitranslmed.aal1803

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors, some rights reserved

This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 2 — Emergence of Bio-AMS–resistant Mtb strains. (A) Frequency of the emergence of spontaneous resistance in Mtb cultured on standard solid medium in the presence of Bio-AMS at concentrations of 10×, 25×, and 50× the MIC. Isoniazid was used as a control. (B) Activity of Bio-AMS against Mtb strain H37Rv transformed with a multicopy plasmid expressing the Mtb protein Rv3406. The Mtb H37Rv strain contained either the vector control (pTE-mcs) or the Rv3406 expression plasmid pGMEHPtb38- rv3406. One spontaneously resistant isolate (Bio-AMS-5R1) was included as a control. (C) Saturation curve used to determine the kinetic parameters for Bio-AMS oxidation by the Mtb protein Rv3406. Left: Data of initial velocity (v0) versus concentration of Bio-AMS were fitted by nonlinear regression to the Michaelis-Menten equation. Right: Time course in milliabsorbance units (mAU) showing Rv3406-catalyzed formation of UV active metabolite 3 at 254 nm, as monitored by high-performance liquid chromatography.