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. 2018 Aug 21;40(5):2573–2583. doi: 10.3892/or.2018.6662

Figure 3.

Figure 3.

Effects of HOXA10-AS-knockdown on glioma cell apoptosis. HOXA10-AS was stably silenced in A172 and U251 cells by transfection of shRNA. (A and B) Flow cytometric analysis demonstrated that the cell apoptosis rate was increased in HOXA10-AS-knockdown glioma cells. Error bars represent the standard error of the mean of three independent experiments. *P<0.05 by Student's t-test. (C and D) Western blot analysis demonstrating the downregulation of total caspase-3 and Bcl-2 protein expression, and the upregulation of cleaved caspase-3 protein in HOXA10-AS-knockdown glioma cells. β-actin served as a loading control.