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. 2018 Sep 12;40(5):2637–2647. doi: 10.3892/or.2018.6702

Table II.

Primer sequences for reverse transcription-quantitative polymerase chain reaction.

Genes Forward primer (5′-3′) Reverse primer (5′-3′) Product length, bp Temperature, °C
FAP ATGAGCTTCCTCGTCCAATTCA AGACCACCAGAGAGCATATTTTG 215 58
IFI27 TGCTCTCACCTCATCAGCAGT CACAACTCCTCCAATCACAACT 115 60
LAMC2 GACAAACTGGTAATGGATTCCGC GACAAACTGGTAATGGATTCCGC 98 60
MMP1 AAAATTACACGCCAGATTTGCC GGTGTGACATTACTCCAGAGTTG 82 58
SPINK5 ATAGCCACAGTGTCAGTGCTT TGTTGCGTAAGGCTTGTGTTC 202 58
ZNF662 CAAACCTGATTCGTCACCAGA TGTTGCGTAAGGCTTGTGTTC 100 60
ACTB AGCGAGCATCCCCCAAAGTT GGGCACGAAGGCTCATCATT 285 60

FAP, fibroblast activation protein α; IFI27, interferon α inducible protein 27; LAMC, laminin subunit γ2; MMP1, matrix metallopeptidase 1; SPINK5, serine peptidase inhibitor Kazal-type 5; ZNF662, zinc finger protein 662; ACTB, β-actin.