Table II.
Genes | Forward primer (5′-3′) | Reverse primer (5′-3′) | Product length, bp | Temperature, °C |
---|---|---|---|---|
FAP | ATGAGCTTCCTCGTCCAATTCA | AGACCACCAGAGAGCATATTTTG | 215 | 58 |
IFI27 | TGCTCTCACCTCATCAGCAGT | CACAACTCCTCCAATCACAACT | 115 | 60 |
LAMC2 | GACAAACTGGTAATGGATTCCGC | GACAAACTGGTAATGGATTCCGC | 98 | 60 |
MMP1 | AAAATTACACGCCAGATTTGCC | GGTGTGACATTACTCCAGAGTTG | 82 | 58 |
SPINK5 | ATAGCCACAGTGTCAGTGCTT | TGTTGCGTAAGGCTTGTGTTC | 202 | 58 |
ZNF662 | CAAACCTGATTCGTCACCAGA | TGTTGCGTAAGGCTTGTGTTC | 100 | 60 |
ACTB | AGCGAGCATCCCCCAAAGTT | GGGCACGAAGGCTCATCATT | 285 | 60 |
FAP, fibroblast activation protein α; IFI27, interferon α inducible protein 27; LAMC, laminin subunit γ2; MMP1, matrix metallopeptidase 1; SPINK5, serine peptidase inhibitor Kazal-type 5; ZNF662, zinc finger protein 662; ACTB, β-actin.