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. 2018 Sep 6;40(5):2608–2618. doi: 10.3892/or.2018.6687

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — KLF5 promoted the invasive and metastatic potential of thyroid cancer cells in vitro. (A) Cell migration/invasion of SW579/scrRNA, SW579/siKLF5#1 and SW579/siKLF5#2 was analyzed using a Transwell assay. (B) Cell migration/invasion of B-CPAP/NC and B-CPAP/KLF5 cells was assessed using a Transwell assay. (C) Western blot analysis of lysates from SW579/scrRNA, SW579/siKLF5#1 and SW579/siKLF5#2 cells using anti-E-cadherin, anti-vimentin, anti-Twist1 and anti-fibronectin and (D) the western blot analysis results quantified. (E) Immunostaining of phalloidin (F-actin) in SW579/scrRNA, SW579/siKLF5#1 and SW579/siKLF5#2 cells. (F) Western blot analysis of lysates from B-CPAP/NC and B-CPAP/KLF5 cells using the indicated antibodies and (G) the western blot analysis results quantified. (H) Immunostaining of phalloidin (F-actin) in B-CPAP/NC and B-CPAP/KLF5 cells. KLF5, Kruppel-like factor 5; scrRNA, scrambled RNA; si-, small interfering RNA; NC, negative control; Twist1, Twist family BHLH transcription factor 1.