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. 2018 Sep 10;40(5):2886–2895. doi: 10.3892/or.2018.6693

Figure 3.

Figure 3.

SH suppresses the expression of the DSBR protein Ku80 and Rad51 at the transcriptional level. (A) IB of HeLa cell lysates for Ku80 and Rad51. β-actin was probed as a loading control. (B and C) Relative mRNA level of Ku80 and Rad51 in control cells and cells treated by SH, IR and SH plus IR, respectively. Total RNA was extracted from HeLa cells following various treatments, and the mRNA expression was examined by RT-PCR. Relative levels of Ku80 and Rad51 mRNA were normalized using GAPDH as an internal control (*P<0.05). (D) IB of HeLa cell lysates for pChk2 T68 and pChk1 S345. β-actin was probed as a loading control. SH, sinomenine hydrochloride; DSBR, DSB repair; double-strand break.