Figure 4.

TAMs secreting EGF and TGF-β depend on HNSCC cells. (A and B) Detection of EMT-associated cytokines, (A) EGF and (B) TGF-β, in TAMs co-cultured with Cal27 cells by reverse transcription-quantitative polymerase chain reation. M0 macrophages were used as the control. (C) EGF and (D) TGF-β cytokines in the co-culture system were detected by ELISA. Data are expressed as the mean ± standard error of the mean. *P<0.05, **P<0.01 and ***P<0.001, as indicated. TAMs, tumor associated macrophages; EMT, epithelial to mesenchymal transition; TGF-β, transforming growth factor-β; EGF, epidermal growth factor.