Figure 1.

Experimental overview. (A) Structures of amifostine (Am) (left) and its dephosphorylated active metabolite, WR-1065 (WR) (right). (B) Unilateral retroductal (Ret) injection of Texas Red solution (50 µg) into the left submandibular gland (SMG) is detected using IVIS imaging (right) and noninjected control (left). (C) Experimental timeline. Injections of Am or WR were completed less than 30 min prior to administration of 15 Gy IR. Tissue was harvested at 48 h, 2 wk, 6 wk, or 12 wk (timeline not to scale). IP, intraperitoneal; IR, irradiation; Ret, retroductal; WR, WR-1065.