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. 2018 Apr 27;97(11):1236–1243. doi: 10.1177/0022034518770605

Figure 2.

Figure 2.

tsRNA-000794 and tsRNA-020498 induced growth inhibition in Fusobacterium nucleatum. (A) Different concentrations of tsRNA-000794, tsRNA-020498, tsRNA-016926, tsRNA-018570, piRNA-016792, and piRNA-006465 were added to F. nucleatum cultures. Cultures were incubated overnight at 37 ºC under anaerobic condition before OD600 was measured. The growth inhibition is expressed as percentage of absorbance to that of the negative control (no addition of tRF). (B) tsRNA-000794 and tsRNA-020498 were pretreated with RNase A before being added to the F. nucleatum culture, and impact on bacterial growth was similarly monitored. tsRNA-000794 (100 µM), tsRNA-020498 (100 µM), piRNA-016792 (100 μM), or scrambled control RNA (100 μM) was added to F. nucleatum (C) and Streptococcus mitis (D), and the growth curves were measured. Assays were performed in triplicates, and mean ± SEM values are shown. ns, P > 0.05; *P < 0.05; **P < 0.01; ****P < 0.0001. All the columns without “ns” or stars are nonsignificant according to statistical tests. piRNA, PIWI-interacting RNA; tsRNA, transfer RNA–derived small sRNA; tRF, tRNA-derived fragment.