TABLE 1.

GLUCOCORTICOIDS RESPONSIVENESS OF THE Nat2*-HRE TRANSFECTED INTO CV1 CELLS

CAT Constructs	rGR	Butylated Chloramphenicol (× 104 dpm) ± SEM
		Hormone Treatment
		No Hormone	Dexamethasone	DHT	β-Estradiol
0 × HRE	+	0.63 ± 0.03	0.31 ± 0.08	0.52 ± 0.08	0.78 ± 0.07
1 × HRE	+	8.70 ± 0.44	4.21 ± 0.30	nd	nd
2 × HRE	−	6.42 ± 0.42	8.52 ± 0.76	nd	nd
2 × HRE	+	3.86 ± 0.58	41.37 ± 2.94	3.98 ± 0.16	5.06 ± 0.51
3 × HRE3	+	9.41 ± 0.63	111.00 ± 5.27	nd	nd

Cells were cotransfected with 100 ng of rat glucocorticoid receptor (GR) and 2 μg of target DNA (0 × HRE, 1 × HRE, 2 × HRE, or 3 × HRE3). Subsequent treatment was for 72 h with no hormone, 340 nM DHT, 100 nM dexamethasone, or 100 nM β-estradiol. Cells were also transfected in the presence or absence of GR. CAT activities measured as the formation of butylated chloramphenicol were normalized to total amount of protein (mg) and are expressed as average (× 10⁴ dpm) of triplicate samples ± SEM. nd, not determined.