Table 3.
Subset of genes with significant differential transcript levels in D5A during nitrogen-deprived condition (5 mM, − N) compared to nitrogen-repleted condition (35 mM, + N) in time points of T1, T2, and T3, as well as D5A compared to BY4741
| Name | D5A (− N/+ N) (Log2) | D5A/BY4741 (Log2) | Function |
|---|---|---|---|
| FA/TAG synthesis | |||
| OLE1 | 1.2 | 4.5 | Delta(9) fatty acid desaturase |
| ELO3 | 1.5 | 0.2 | Elongase |
| PIS1 | 1.1 | − 0.3 | Phosphatidylinositol synthase |
| POX1 | − 1.4 | 2.4 | Fatty-acyl coenzyme A oxidase |
| FAA1 | − 1.4 | 0.8 | Long-chain fatty-acyl-CoA synthetase |
| N metabolism | |||
| GLT1 | 1.6 | 1.0 | NAD(+)-dependent glutamate synthase (GOGAT) |
| GLN1 | 1.9 | 0.00 | Glutamine synthetase (GS) |
| GDH1 | 1.4 | − 0.3 | NADP(+)-dependent glutamate dehydrogenase |
| GDH2 | 0.6 | 0.5 | NAD(+)-dependent glutamate dehydrogenase |
| AA metabolism | |||
| ARO8 | 3.4 | − 1.5 | Aromatic aminotransferase I |
| LEU2 | 2.4 | 6.9 | Beta-isopropylmalate dehydrogenase (IMDH) |
| CHA1 | 2.7 | − 3.6 | Catabolic l-serine (l-threonine) deaminase |
| BAT1 | 3.3 | − 2.5 | Mitochondrial branched-chain amino acid (BCAA) aminotransferase |
| ASN1 | 2.4 | − 3.1 | Asparagine synthetase |
| EMP | |||
| HXK2 | 2.9 | − 0.3 | Hexokinase isoenzyme 2 |
| TDH1 | − 3.8 | 1.4 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
| HXK1 | − 3.2 | 1.4 | Hexokinase isoenzyme 1 |
| GLK1 | − 3.0 | 1.9 | Glucokinase |
| GPM1 | − 2.1 | − 0.3 | Tetrameric phosphoglycerate mutase |
| FBP1 | − 2.1 | 1.6 | Fructose-1,6-bisphosphatase |
| PYK2 | − 2.1 | − 0.6 | Pyruvate kinase |
| PGK1 | − 1.9 | − 0.1 | 3-Phosphoglycerate kinase |
| PPP | |||
| TKL1 | 2.4 | − 0.1 | Transketolase |
| SOL3 | 1.4 | − 326 | 6-Phosphogluconolactonase |
| GND1 | 1.1 | 0.3 | 6-Phosphogluconate dehydrogenase (decarboxylating) |
| TKL2 | − 3.3 | 3.0 | Transketolase |
| TAL1 | − 2.1 | 0.3 | Transaldolase |
| ZWF1 | − 1.7 | − 1.0 | Glucose-6-phosphate dehydrogenase (G6PD) |
| TCA cycle | |||
| PYC1 | − 2.0 | 0.3 | Pyruvate carboxylase isoform |
| CIT1 | − 3.3 | 1.1 | Citrate synthase |
| CIT3 | − 2.6 | − 0.3 | Dual specificity mitochondrial citrate and methylcitrate synthase |
| ACO1 | − 1.2 | 2.5 | Aconitase |
| SDH1 | − 1.5 | 1.8 | Flavoprotein subunit of succinate dehydrogenase |
| MDH1 | − 2.3 | 0.8 | Mitochondrial malate dehydrogenase |
| SDH2 | − 1.6 | 1.9 | Iron–sulfur protein subunit of succinate dehydrogenase |
| FUM1 | − 1.0 | 1.3 | Fumarase |
| KGD1 | − 1.5 | 1.0 | mitochondrial α-ketoglutarate dehydrogenase complex subunit |
| KGD2 | − 1.1 | 1.7 | Dihydrolipoyl transsuccinylase |
| ICL1 | − 1.4 | 1.4 | Isocitrate lyase |
| Other | |||
| YAT1 | − 4.1 | 0.5 | Outer mitochondrial carnitine acetyltransferase |
| CIT2 | − 4.9 | − 0.4 | Citrate synthase |
| ICL1 | − 1.4 | 1.4 | Isocitrate lyase |
| ACS1 | − 3.5 | 2.1 | Acetyl-coA synthetase isoform |
| PDC6 | 1.5 | 0.7 | Minor isoform of pyruvate decarboxylase |
| ADH4 | − 3.3 | 4.0 | Alcohol dehydrogenase isoenzyme type IV |
| ALD6 | − 1.2 | 1.3 | Cytosolic aldehyde dehydrogenase |
| GPP1 | 1.2 | − 0.5 | Constitutively expressed dl-Glycerol-3-phosphate phosphatase |
| GPP2 | 1.5 | − 0.7 | dl-Glycerol-3-phosphate phosphatase involved in glycerol biosynthesis |
All transcriptional differences are shown as the mean of duplicate log2-based values