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Gel-shift analysis of HIF3α. Radiolabeled oligonucleotide probe containing the HRE core sequence TACGTG was incubated with HIF3α and/or ARNT in the absence or presence of preimmune (PI) or immune (Imm) anti-ARNT antibody as described in Materials and Methods. For competition experiments, 400-fold of either wild-type (TACGTG) or mutant (AACGTG or GACGTG) oligonucleotides was added into each reaction. Specific (HIF3α/ARNT) and nonspecific (NS) complexes are marked.
