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. 2018 Sep 15;24:6480–6488. doi: 10.12659/MSM.910050

Figure 3.

Figure 3

NMP significantly inhibits the LPS-induced increase of COX-2 and iNOS expression. ATDC5 cells were pre-treated with various concentrations of NMP (0, 5, and 10 nM) and then treated with 5 μg/ml LPS. The protein (A) and mRNA (B, C) levels of COX-2 and iNOS was determined using Western blot and qRT-PCR, respectively. Control: cells without any treatment; LPS: cells were treated with 5 μg/ml LPS for 6 h at 37°C; LPS+NMP5: cells were treated with 5 mM NMP for 48 h at 37°C and then 5 μg/ml LPS for 6 h at 37°C; LPS+NMP10: cells were treated with 10 mM NMP for 48 h at 37°C and then 5 μg/ml LPS for 6 h at 37°C. ** p<0.01 vs. control; #, ## p<0.05, 0.01 vs. LPS group.