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. 2017 Aug 17;22(8):1363. doi: 10.3390/molecules22081363

Figure 1.

Figure 1

The effects of SM70EE on cell viability and neuronal death in HT-22 cells. (A) Cell viability was measured by a MTT assay. The experiments were performed in hexa-plicate; (B) HT-22 cells were treated with up to 10 μΜ TMT. Cell lysates were subjected to western blot analysis to analyze to the expression of PARP; (C) HT-22 cells were pre-treated 50 and 100 μg/mL SM70EE for 4 h before treatment with 10 μM TMT for 24 h. Cell lysates were subjected to western blot analysis to probe the expression of PARP. The protein expression level was quantified using Image J software and normalized against α-tubulin and control. Results were analyzed by one-way analysis of variance (ANOVA) and Duncan’s multiple range tests. The p-value in the multiple comparison results (e.g., a, b, and c) indicate significant differences among the groups (p < 0.05).