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. 2017 Aug 17;22(8):1363. doi: 10.3390/molecules22081363

Figure 2.

Figure 2

SM70EE inhibits ROS production in TMT-induced neurotoxicity in HT-22 cells. (A) HT-22 cells were pretreated 50 and 100 μg/mL SM70EE for 4 h before treatment with 10 μM TMT for 24 h. ROS production was measured by a DCF-DA assay. The experiments were performed in hexa-plicate; (B) HT-22 cells were pre-treated 50 and 100 μg/mL SM70EE for 4 h before treatment with 10 μM TMT for 24 h. Cells lysates were subjected to western blot analysis to measure the levels of HO-1, NOX4, and SOD2. The protein expression level was quantified using Image J software and normalized against α-tubulin and control. Results were analyzed by one-way ANOVA and Duncan’s multiple range tests. The p-value in the multiple comparison results (e.g., a, b, c, and d) indicate significant differences among the groups (p < 0.05).