Table 1.

Box–Behnken design (RSM 3-level-3-factor-1 replicate) and observed experimental total phenol content (TPC), total monomeric anthocyanin (TMA), and radical scavenging activity (ABTS and DPPH) data from Brosimum alicastrum left-extract.

Run	Block	X1		X2		X3		Y1	Y2	Y3	Y4
		Amplitude (%)		Time (min)		T° (±0.5 oC)		TPC (mg GAE/g)	TMA (mg CyE)/100 g	ABTS (μmol TE/g)	DPPH (μmol TE/g)
1	1	−1	40	−1	10	0	30	35.67	3.76	27.57	31.04
2	1	1	80	−1	30	0	30	41.71	6.16	30.48	41.22
3	1	−1	40	1	30	0	30	41.82	7.41	31.47	42.66
4	1	1	80	1	30	0	30	42.86	6.99	35.06	45.54
5	1	−1	40	0	20	−1	28	39.03	5.22	29.74	38.01
6	1	1	80	0	20	−1	28	44.52	13.99	36.51	65.74
7	1	−1	40	0	20	1	32	42.65	8.14	33.59	43.60
8	1	1	80	0	20	1	32	40.82	5.53	29.95	39.12
9	1	0	60	−1	10	−1	28	34.85	3.44	22.96	25.44
10	1	0	60	1	30	−1	28	34.30	2.09	21.56	17.24
11	1	0	60	−1	10	1	32	33.97	1.88	19.50	14.23
12	1	0	60	1	30	1	32	37.82	4.70	26.54	36.99
13	1	0	60	0	20	0	30	36.68	3.24	25.86	31.66
14	1	0	60	0	20	0	30	38.79	4.80	27.86	38.60
15	1	0	60	0	20	0	30	36.71	3.97	26.24	32.95
1	2	−1	40	−1	10	0	30	36.13	4.35	27.88	35.92
2	2	1	80	−1	30	0	30	41.73	10.14	32.28	42.71
3	2	−1	40	1	30	0	30	42.31	10.51	37.56	50.03
4	2	1	80	1	30	0	30	44.36	10.09	35.34	51.42
5	2	−1	40	0	20	−1	28	39.58	5.51	31.81	45.28
6	2	1	80	0	20	−1	28	45.18	15.17	36.93	67.27
7	2	−1	40	0	20	1	32	43.06	10.50	38.79	44.13
8	2	1	80	0	20	1	32	41.75	6.27	31.42	39.58
9	2	0	60	−1	10	−1	28	35.39	5.07	23.23	28.48
10	2	0	60	1	30	−1	28	34.33	2.09	26.29	20.29
11	2	0	60	−1	10	1	32	34.11	2.47	21.04	17.28
12	2	0	60	1	30	1	32	37.91	6.02	26.97	39.01
13	2	0	60	0	20	0	30	36.93	3.68	25.98	36.50
14	2	0	60	0	20	0	30	39.47	5.10	30.53	38.88
15	2	0	60	0	20	0	30	38.48	4.56	26.88	33.16

TPC = Total phenolic content; TMA = Total monomeric anthocyanin; ABTS = 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid; DPPH = 2,2-diphenyl-1-picrylhydrazyl. Amplitude of 40%, 60% and 80% correspond to sonication power of 28 W/cm²; 51 W/cm², 74 W/cm², respectively.