Table 1.
Design and its results for the surface methodology experiment.
| Runs | X1 | X2 | X3 | Y1 (Betulin Conversion Rate, %) | Y2 (Betulinic Acid Yield, %) |
|---|---|---|---|---|---|
| 1 | 0 | 1 | −1 | 21.58 | 3.33 |
| 2 | −1 | 0 | −1 | 61.76 | 4.14 |
| 3 | 0 | −1 | −1 | 20.06 | 16.93 |
| 4 | 0 | 0 | 0 | 30.28 | 6.33 |
| 5 | 1 | 0 | −1 | 35.87 | 9.10 |
| 6 | −1 | 1 | 0 | 57.02 | 4.81 |
| 7 | −1 | −1 | 0 | 58.74 | 7.63 |
| 8 | 0 | 1 | 1 | 33.48 | 5.16 |
| 9 | 1 | −1 | 0 | 45.52 | 14.67 |
| 10 | −1 | 0 | 1 | 57.25 | 3.23 |
| 11 | 0 | −1 | 1 | 38.83 | 13.83 |
| 12 | 1 | 1 | 0 | 41.66 | 7.96 |
| 13 | 0 | 0 | 0 | 37.10 | 7.87 |
| 14 | 0 | 0 | 0 | 32.05 | 7.56 |
| 15 | 0 | 0 | 0 | 36.89 | 7.06 |
| 16 | 0 | 0 | 0 | 35.12 | 7.32 |
| 17 | 1 | 0 | 1 | 39.87 | 14.75 |
X1: transformation time (h); X2: the concentration of betulin (μM); X3: the concentration of nicotinamide adenine dinucleotide phosphate (NADPH) (mM). X1 = (x1 − 6)/3, X2 = (x2 − 80)/40, X3 = (x3 − 1)/1.