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HPTLC (High Performance Thin Layer Chromatography) bioautographic assay performed for antibacterial activity on S. aureus, of M. splendens essential oil. Rf: retention factor: it represents the ratio between the migration distance of a substance and the migration distance of the solvent front. Rf composition is reported in Table 1. (A) HPTLC derivatized with vanillin-phosphoric acid reagent. (B) HPTLC performed for antibacterial activity on S. aureus.
