Table 1.

Chemical composition of M. splendens essential oil and its characterization in term of active antioxidant and antibacterial compounds isolated respectively from DPPH-HPTLC (1,1-diphenyl-2-picrylhydrazil-high performance thin layer chromatography) and HPTLC bioautography assays.

					Antioxi. F. 5		Antibact. F. 6
No.	% Area 1	Component 2	AI Exp 3	AI Lit 4	Rf = 0.9	Rf = 0.45–0.6		Rf = 0.7
1	2.08 ± 0.10	α-pinene	928	939
2	0.11 ± 0.01	β-pinene	972	979
3	0.15 ± 0.02	α-cubebene	1350	1351	1.39
4	0.51 ± 0.03	α-copaene	1375	1377	4.21
5	0.11 ± 0.02	β-elemene	1387	1391	4.39
6	4.21 ± 0.15	β-caryophyllene	1409	1419	36.23
7	0.31 ± 0.02	trans-α-bergamotene	1430	1435	3.15
8	0.45 ± 0.03	α-caryophyllene	1449	1455	3.94
9	0.87 ± 0.06	trans-β-farnesene	1453	1457	7.81
10	0.65 ± 0.04	germacrene D	1475	1485	4.62
11	0.10 ± 0.01	cis-β-guaiene	1485	1493	1.54
12	0.17 ± 0.02	viridiflorene	1490	1497	1.43
13	0.11 ± 0.01	α-muurolene	1497	1499	1.27
14	0.59 ± 0.03	α-bisabolene	1503	1507	1.24
15	0.24 ± 0.02	cis-γ-bisabolene	1506	1515	8.33
16	0.53 ± 0.04	δ-cadinene	1513	1523	4.93
17	0.16 ± 0.01	trans-calamenene	1518	1529	1.66
18	1.03 ± 0.09	trans-γ-bisabolene	1523	1531	10.04
19	67.81 ± 2.10	trans-nerolidol	1562	1563		100
20	0.15 ± 0.01	caryophyllene oxide	1580	1583
21	Traces	β-cedren-9-one	1630	1631				87.34
22	17.51 ± 1.01	α-bisabolol	1690	1686
Total identified	97.84

¹ Relative peak areas ± SEM (standard error media), calculated by GC-FID; ² Components are listed in order of elution and their nomenclature is in accordance of the NIST (National Institute of Standards and Technology) library; ³ AI exp: arithmetic indices calculated on a Varian VF-5ms column; ⁴ AI lit: arithmetic indices [11]; ⁵ Antioxi. F.: fraction of compounds responsible for antioxidant activity. ⁶ Antibact. F.: fraction of compounds responsible for antibacterial activity.