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. 2017 Jul 12;22(7):1160. doi: 10.3390/molecules22071160

Figure 5.

Figure 5

Effects of TBEs on intracellular lipid accumulation and TG content during adipocyte differentiation. Scheme of the 3T3-L1 preadipocyte differentiation experiment (a). 3T3-L1 cells were treated with 100 µg/mL of TBE-50 or TBE-70, and incubated for 2, 5, or 7 days (d2 to d9). On Day 7 (d9), change of adipocyte differentiation was presented with Oil Red O staining (b). Intracellular lipid content (c) was stained with oil-red O dye, and dissolved the stained oil droplets with isopropanol and quantified by spectrophotometric analysis. Representative cell images were captured at 200× magnification. 3T3-L1 cells were treated with 0 (MDI treated control), 1, 50, and 100 µg/mL of TBE-50 and TBE-70, and incubated for 7 days. Intracellular TG content (d) was determined using enzymatic colorimetric methods. MDI, medium containing 3-isobutyl-1-methylxanthine, dexamethasone and insulin. Values are expressed as mean ± SE (n = 3) of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.01 vs. MDI-treated control. # p < 0.05 vs. TBE-50.