Table 1.
Lactose Kd of hGal1, hGal7, hGal8NTD, hGal8CTD, NTD of full-length galectin-8 (hGal8full(NTD)) and CTD of full-length galectin-8 (hGal8full(CTD)) determined by four independent biophysical tools expressed in μM.
| NMR (CSP) | NMR (TITAN) | Intrinsic Fluorescence | BLI | ITC | |
|---|---|---|---|---|---|
| hGal1 | 104 ± 6 | 112 ± 1 | 98 ± 8 | 110 ± 38 | 209 ± 13 |
| hGal7 | 397 ± 14 | 352 ± 9 | 331 ± 6 | 347 ± 180 | 400 ± 2 |
| hGal8NTD | 84 ± 9 | 86 ± 3 | 88 ± 2 | 89 ± 26 | 93 ± 1 |
| hGal8CTD | 664 ± 59 | 950 ± 10 | 772 ± 56 | 873 ± 526 | 893 ± 25 |
| hGal8full(NTD) | 128 ± 9 | 167 ± 7 | — | — | — |
| hGal8full(CTD) | 1478 ± 69 | 1186 ± 11 | — | — | — |