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LC-MS profile (BPC) of a representative Fragmin® sample (Frag-5) following digestion with heparinase III. Mass signals assignment of the main components is reported. Components identified in the parent sample, before the enzymatic digestion, are indicated in italic font. LC elution conditions: isocratic at 10% B for 5 min, linear gradient from 10% to 50% B in 55 min, linear gradient from 50% B to 90% B in 80 min, followed by column washing and reconditioning.
